Restriction fragment length polymorphism markers were the first DNA-based genetic markers developed. The size of eukaryotic genomes hindered a simple identification of genetic variations in specific genes or sequences. The capability of complementary base pairing to reveal polymorphisms in sequences homologous to the probe enabled the development of methods to […]
Immunoglobulins The glycoproteins produced by B-lymphocytes effector cells or plasma cells against specific antigens are known as Immunoglobulins or Antibodies. Antibodies are made up of multiple immunoglobulin domains that are composed of a polypeptide chain that folds into a series of antiparallel β-pleated strands and these strands are arranged into […]
The study of stem cells has produced more enthusiasm and promise than any other discovery in the field of regenerative medicine. The amazing capacity of stem cells to differentiate into multiple cell types within the body makes them essential for healing and replacing harmed or sick tissues. In this article, […]
Sanger sequencing, the primary approach of DNA sequencing since its inception in the 1970s, was used to complete the sequencing of the human genome in 2003. The need for rapid, cost-effective, and reliable analysis of vast amounts of genomic DNA is driving a massive increase in the demand […]
Malaria, a mosquito-borne infectious disease, continues to pose a significant threat to human health in many parts of the world. Despite advancements in medicine and global efforts to combat the disease, malaria remains a leading cause of illness and mortality, particularly in tropical and subtropical regions. This article explores the […]
Enzyme-linked immunosorbent assay, commonly known as ELISA or EIA, a technique that depends on an enzyme. Both antigens as well as antibody can be detected by conjugating an enzyme with an antibody that reacts with a colourless substrate but generate a colour reaction. The colourless substrate that generates colour upon […]
Mitochondria is an inevitable cell organelle necessary for survival. They are the primary source for the development of the majority of the Adenosine Triphosphate (ATP) which acts as the frontline form of energy in the body and involves cellular respiration. Mitochondria are always referred to as the powerhouse of cells. […]
Image Source: www.selectscience.net In the field of molecular biology, the visualization and analysis of DNA and proteins are crucial for various research endeavours. Gel electrophoresis has long been the go-to method for separating and analyzing nucleic acids and proteins. However, the process of visualizing and quantifying the separated molecules was […]
Image: newcomersupply.com Safranin staining is a widely used technique in the field of biology and histology that allows researchers to visualize and study various biological structures with enhanced contrast. This staining method utilizes safranin, a red dye derived from saffron, which selectively binds to specific cellular components, providing […]
Crystal structure of Streptococcus thermophilus Cas9 in complex with AGGA PAM. Source: RCSB PDB Clustered Regularly Interspaced Short Palindromic Repeats or CRISPR is a new-generation gene editing system. This technique was adapted from the self-defensive mechanism of the bacteria. CRISPR is a repetitive DNA sequence that was observed in […]
