The powerful analytical method, high-performance liquid chromatography (HPLC), is employed in many scientific disciplines, including chemistry, pharmacology, biochemistry, and environmental studies.
Biotechnology
The basis of the randomly amplified polymorphic DNA (RAPD) technique is differential PCR amplification of genomic DNA. It uses short random oligonucleotide sequences (usually 10 bases long) to infer DNA polymorphisms caused by “rearrangements or deletions at or between oligonucleotide primer binding sites in the genome.”
Trisomy 21 another name for Down syndrome is a genetic disorder that impacts millions of people worldwide. It bears the name of British surgeon John Langdon Down, who initially identified the illness in 1866. An additional copy of chromosome 21 causes developmental and intellectual difficulties as well as distinctive talents […]
Restriction fragment length polymorphism markers were the first DNA-based genetic markers developed. The size of eukaryotic genomes hindered a simple identification of genetic variations in specific genes or sequences. The capability of complementary base pairing to reveal polymorphisms in sequences homologous to the probe enabled the development of methods to […]
Immunoglobulins The glycoproteins produced by B-lymphocytes effector cells or plasma cells against specific antigens are known as Immunoglobulins or Antibodies. Antibodies are made up of multiple immunoglobulin domains that are composed of a polypeptide chain that folds into a series of antiparallel β-pleated strands and these strands are arranged into […]
Crystal structure of Streptococcus thermophilus Cas9 in complex with AGGA PAM. Source: RCSB PDB Clustered Regularly Interspaced Short Palindromic Repeats or CRISPR is a new-generation gene editing system. This technique was adapted from the self-defensive mechanism of the bacteria. CRISPR is a repetitive DNA sequence that was observed in […]
The Human Genome Project was a major scientific study in the history of life-sciences. This project was lead to many biological discoveries which can aid the future research and developments. The aim of the project was to study all the DNA or Genomes of the selected set of organisms. This […]
Nucleic Acids Nucleic acids, the building block of genome it can be either DNA or RNA. The genome is the entire set of genetic material in a haploid cell. There are 46 or 23 pair of chromosomes are present in a cell. The genome resides mainly in the nucleus […]
Transcription is the process by which the genetic information stored in DNA is copied into RNA. This process is essential for the expression of genes and the synthesis of proteins. Transcription occurs differently in eukaryotes and prokaryotes due to differences in the organization of their genetic material. Transcription in Prokaryotes […]
Blood, “The River of Life” as it supports life. It contains the nutrients and oxygen that support living organisms. The cells in the blood are generally synthesized in the bone marrow by a process called haematopoiesis. Blood contains three types of blood cells, namely Erythrocytes or RBC, Leukocytes or […]
